Part:BBa_I746203:Design

Intermediate for Outer Membrane Permeability Device

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 256
  Illegal AgeI site found at 622
  Illegal AgeI site found at 1249
  Illegal AgeI site found at 1767
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Removal of an EcoR1 site by site directed mutagenesis.

Source

The L8T FepA mutant was kindly supplied to us in a plasmid by Professor P.E. Klebba.